9.5 Thermostability Engineering

Thermostability Engineering (Module 9: The Optimizer). Key concept: Resistance to heat-induced unfolding. Apply the five stabilisation strategies: cavity filling, salt bridges, loop rigidification, disulfides and surface charge.

• Apply the five stabilisation strategies: cavity filling, salt bridges, loop rigidification, disulfides and surface charge
• Raise a melting temperature without damaging the active site
• Match each strategy to the defect type it addresses
• Treat a summed ΔTm as an optimistic ceiling, because real mutations combine sub-additively