6.4 The Validation Pipeline

The Validation Pipeline (Module 6: The Experimentalist). Key concept: Expression to characterization. Trace the path from gene synthesis through expression, purification and verification.

• Trace the path from gene synthesis through expression, purification and verification
• Interpret the standard assays: SDS-PAGE for size, SEC for oligomeric state, mass spectrometry for identity, circular dichroism for secondary structure
• Recognise what success and failure look like at each step
• Explain why no computational prediction substitutes for this pipeline