6.1 Expression Systems

Expression Systems (Module 6: The Experimentalist). Key concept: E. coli, yeast, insect, mammalian. Compare the four main expression systems: E. coli, yeast, insect and mammalian cells.

• Compare the four main expression systems: E. coli, yeast, insect and mammalian cells
• Weigh the trade-offs between speed, cost, yield and post-translational modification
• Choose a system from what the protein actually requires
• Recognise that this choice can decide a design’s fate before any science happens