6.2 The Solubility Problem

The Solubility Problem (Module 6: The Experimentalist). Key concept: Aggregation and inclusion bodies. Explain why a designed protein may aggregate rather than fold.

• Explain why a designed protein may aggregate rather than fold
• Identify exposed hydrophobic patches as the primary aggregation driver
• Recognise charge imbalance and insufficient surface polarity as additional risk factors
• Predict solubility from surface properties before committing to synthesis