Module 3: The Architect

Global Folding

Understand how secondary structures pack into domains and tertiary structure.

• Explain why the hydrophobic core is the primary thermodynamic driving force for folding
• Judge packing quality and recognise cavities as a destabilising defect
• Apply the geometric requirements for a viable disulfide bond
• Diagnose buried charges and exposed hydrophobic patches from a residue distribution
• Identify domain boundaries and explain why they matter for redesign
• Repair several independent structural defects in one protein and predict the consequences