8.3 Hotspot Definition

Hotspot Definition (Module 8: The Creator). Key concept: Identifying target residues for binder design. Select hotspot residues on a target for binder design.

• Select hotspot residues on a target for binder design
• Weigh exposure, conservation, proximity to the functional interface and spatial clustering
• Explain why clustering constrains a hotspot set as hard as conservation does: one binder contacts one patch
• Apply what Modules 4 and 5 taught to a real design decision