# 9.5 Thermostability Engineering

> Thermostability Engineering (Module 9: The Optimizer). Key concept: Resistance to heat-induced unfolding. Apply the five stabilisation strategies: cavity filling, salt bridges, loop rigidification, disulfides and surface charge.

Thermostability Engineering (Module 9: The Optimizer). Key concept: Resistance to heat-induced unfolding. Apply the five stabilisation strategies: cavity filling, salt bridges, loop rigidification, disulfides and surface charge.

- Apply the five stabilisation strategies: cavity filling, salt bridges, loop rigidification, disulfides and surface charge
- Raise a melting temperature without damaging the active site
- Match each strategy to the defect type it addresses
- Treat a summed ΔTm as an optimistic ceiling, because real mutations combine sub-additively

## Links

- [Module 9: The Optimizer](https://proteindesign.academy/module/9)
- [Introduction](https://proteindesign.academy/module/9/intro)
- [9.1 Proteins in Motion](https://proteindesign.academy/module/9/1)
- [9.2 B-Factors and Flexibility](https://proteindesign.academy/module/9/2)
- [9.3 Structural Diagnostics](https://proteindesign.academy/module/9/3)
- [9.4 Energy Landscapes](https://proteindesign.academy/module/9/4)
- [9.integration The Guided Repair](https://proteindesign.academy/module/9/integration)
- [The Stability Optimizer](https://proteindesign.academy/module/9/capstone)
- [Use the Tools](https://proteindesign.academy/module/9/tools)