# 8.3 Hotspot Definition

> Hotspot Definition (Module 8: The Creator). Key concept: Identifying target residues for binder design. Select hotspot residues on a target for binder design.

Hotspot Definition (Module 8: The Creator). Key concept: Identifying target residues for binder design. Select hotspot residues on a target for binder design.

- Select hotspot residues on a target for binder design
- Weigh exposure, conservation, proximity to the functional interface and spatial clustering
- Explain why clustering constrains a hotspot set as hard as conservation does: one binder contacts one patch
- Apply what Modules 4 and 5 taught to a real design decision

## Links

- [Module 8: The Creator](https://proteindesign.academy/module/8)
- [Introduction](https://proteindesign.academy/module/8/intro)
- [8.1 What Is De Novo Design?](https://proteindesign.academy/module/8/1)
- [8.2 How Diffusion Models Work](https://proteindesign.academy/module/8/2)
- [8.4 RFdiffusion: The Full Tool](https://proteindesign.academy/module/8/4)
- [The Binder Generator](https://proteindesign.academy/module/8/capstone)
- [Use the Tools](https://proteindesign.academy/module/8/tools)