# Module 8: The Creator

> Generate proteins that have never existed using RFdiffusion and the full design pipeline.

De Novo Design

Generate proteins that have never existed using RFdiffusion and the full design pipeline.

- Explain what de novo design means, and what separates it from redesigning an existing protein
- Describe how a diffusion model builds a backbone by iteratively denoising random residue positions and orientations
- Choose hotspot residues for binder design from conservation, exposure and spatial clustering
- Walk the modular pipeline end to end: target and hotspots → RFdiffusion → ProteinMPNN → AlphaFold2
- Judge generated binders against the published in-silico filters, including the judgement to reject all of them

## Links

- [Introduction](https://proteindesign.academy/module/8/intro)
- [8.1 What Is De Novo Design?](https://proteindesign.academy/module/8/1)
- [8.2 How Diffusion Models Work](https://proteindesign.academy/module/8/2)
- [8.3 Hotspot Definition](https://proteindesign.academy/module/8/3)
- [8.4 Did It Work?](https://proteindesign.academy/module/8/4)
- [8.integration Run RFdiffusion](https://proteindesign.academy/module/8/integration)
- [The Binder Generator](https://proteindesign.academy/module/8/capstone)
- [Use the Tools](https://proteindesign.academy/module/8/tools)
- [Start Here](https://proteindesign.academy/start-here)
- [Tools & Papers](https://proteindesign.academy/tools-and-papers)
- [Continuing Practice](https://proteindesign.academy/continuing-practice)