# 6.4 The Validation Pipeline

> The Validation Pipeline (Module 6: The Experimentalist). Key concept: Expression to characterization. Trace the path from gene synthesis through expression, purification and verification.

The Validation Pipeline (Module 6: The Experimentalist). Key concept: Expression to characterization. Trace the path from gene synthesis through expression, purification and verification.

- Trace the path from gene synthesis through expression, purification and verification
- Interpret the standard assays: SDS-PAGE for size, SEC for oligomeric state, mass spectrometry for identity, circular dichroism for secondary structure
- Recognise what success and failure look like at each step
- Explain why no computational prediction substitutes for this pipeline

## Links

- [Module 6: The Experimentalist](https://proteindesign.academy/module/6)
- [6.1 Expression Systems](https://proteindesign.academy/module/6/1)
- [6.2 The Solubility Problem](https://proteindesign.academy/module/6/2)
- [6.3 Why Designs Fail](https://proteindesign.academy/module/6/3)
- [The Expressibility Audit](https://proteindesign.academy/module/6/capstone)