# 6.2 The Solubility Problem

> The Solubility Problem (Module 6: The Experimentalist). Key concept: Aggregation and inclusion bodies. Explain why a designed protein may aggregate rather than fold.

The Solubility Problem (Module 6: The Experimentalist). Key concept: Aggregation and inclusion bodies. Explain why a designed protein may aggregate rather than fold.

- Explain why a designed protein may aggregate rather than fold
- Identify exposed hydrophobic patches as the primary aggregation driver
- Recognise charge imbalance and insufficient surface polarity as additional risk factors
- Predict solubility from surface properties before committing to synthesis

## Links

- [Module 6: The Experimentalist](https://proteindesign.academy/module/6)
- [6.1 Expression Systems](https://proteindesign.academy/module/6/1)
- [6.3 Why Designs Fail](https://proteindesign.academy/module/6/3)
- [6.4 The Validation Pipeline](https://proteindesign.academy/module/6/4)
- [The Expressibility Audit](https://proteindesign.academy/module/6/capstone)