# 6.1 Expression Systems

> Expression Systems (Module 6: The Experimentalist). Key concept: E. coli, yeast, insect, mammalian. Compare the four main expression systems: E. coli, yeast, insect and mammalian cells.

Expression Systems (Module 6: The Experimentalist). Key concept: E. coli, yeast, insect, mammalian. Compare the four main expression systems: E. coli, yeast, insect and mammalian cells.

- Compare the four main expression systems: E. coli, yeast, insect and mammalian cells
- Weigh the trade-offs between speed, cost, yield and post-translational modification
- Choose a system from what the protein actually requires
- Recognise that this choice can decide a design’s fate before any science happens

## Links

- [Module 6: The Experimentalist](https://proteindesign.academy/module/6)
- [6.2 The Solubility Problem](https://proteindesign.academy/module/6/2)
- [6.3 Why Designs Fail](https://proteindesign.academy/module/6/3)
- [6.4 The Validation Pipeline](https://proteindesign.academy/module/6/4)
- [The Expressibility Audit](https://proteindesign.academy/module/6/capstone)