4.1 Reading a PDB File

Reading a PDB File (Module 4: The Bioinformatician's Toolkit). Key concept: PDB file format & metadata. Explain what the Protein Data Bank is and what depositing a structure commits you to.

• Explain what the Protein Data Bank is and what depositing a structure commits you to
• Read the key PDB record types: HEADER, ATOM, HETATM and the metadata fields
• Extract chains, resolution, method, organism and ligands from a file
• Explain what a B-factor measures and why resolution limits what you can claim
• Compare X-ray crystallography, cryo-EM and NMR by what each one can and cannot resolve