# 11.3 De-immunization

> De-immunization (Module 11: The Healer). Key concept: Identifying and mutating T-cell epitopes. Explain how MHC class II presents peptides: a 13 to 25-residue peptide with a 9-residue core anchored at P1, P4, P6 and P9.

De-immunization (Module 11: The Healer). Key concept: Identifying and mutating T-cell epitopes. Explain how MHC class II presents peptides: a 13 to 25-residue peptide with a 9-residue core anchored at P1, P4, P6 and P9.

- Explain how MHC class II presents peptides: a 13 to 25-residue peptide with a 9-residue core anchored at P1, P4, P6 and P9
- Scan a sequence for high-affinity epitope regions
- Mutate anchor positions to lower an epitope score
- Weigh immunogenicity reduction against the fold: a proline is an excellent anchor disruptor, and a helix is the wrong place for one
- Accept a residual epitope when clearing it would cost the core or the binding site

## Links

- [Module 11: The Healer](https://proteindesign.academy/module/11)
- [Introduction](https://proteindesign.academy/module/11/intro)
- [11.1 Therapeutic Protein Design](https://proteindesign.academy/module/11/1)
- [11.2 Target Selection](https://proteindesign.academy/module/11/2)
- [11.4 Developability](https://proteindesign.academy/module/11/4)
- [11.integration The Drug Pipeline](https://proteindesign.academy/module/11/integration)
- [The Therapeutic Designer](https://proteindesign.academy/module/11/capstone)
- [Use the Tools](https://proteindesign.academy/module/11/tools)