# 10.4 Evaluating Assemblies

> Evaluating Assemblies (Module 10: The Assembler). Key concept: AF-Multimer validation and oligomeric state checks. Interpret AlphaFold-Multimer output: ipTM above 0.8 is confident, 0.6–0.8 is a grey zone, below 0.6 has likely failed.

Evaluating Assemblies (Module 10: The Assembler). Key concept: AF-Multimer validation and oligomeric state checks. Interpret AlphaFold-Multimer output: ipTM above 0.8 is confident, 0.6–0.8 is a grey zone, below 0.6 has likely failed.

- Interpret AlphaFold-Multimer output: ipTM above 0.8 is confident, 0.6–0.8 is a grey zone, below 0.6 has likely failed
- Read cross-chain PAE to judge whether the chains are placed relative to each other
- Test for unintended oligomeric states by running alternative stoichiometries
- Explain why ipTM is a confidence score and not a binding affinity or a free energy

## Links

- [Module 10: The Assembler](https://proteindesign.academy/module/10)
- [Introduction](https://proteindesign.academy/module/10/intro)
- [10.1 Why Multimerize?](https://proteindesign.academy/module/10/1)
- [10.2 Symmetry in Protein Assemblies](https://proteindesign.academy/module/10/2)
- [10.3 Interface Design Principles](https://proteindesign.academy/module/10/3)
- [10.integration The Assembly Blueprint](https://proteindesign.academy/module/10/integration)
- [The Assembly Designer](https://proteindesign.academy/module/10/capstone)
- [Use the Tools](https://proteindesign.academy/module/10/tools)