# Module 10: The Assembler

> Design multi-chain protein complexes with precise control over interfaces and symmetry.

Quaternary Structure

Design multi-chain protein complexes with precise control over interfaces and symmetry.

- Explain the advantages a protein gains by assembling from several chains
- Identify cyclic and dihedral symmetry, and use one designed chain plus a symmetry operation to generate an assembly
- Design an inter-chain interface with adequate buried surface area, contacts and shape complementarity
- Read AlphaFold-Multimer output correctly: ipTM, cross-chain PAE, and the checks for unintended oligomeric states
- Take a symmetric assembly from symmetry choice through interface design to validation

## Links

- [Introduction](https://proteindesign.academy/module/10/intro)
- [10.1 Why Multimerize?](https://proteindesign.academy/module/10/1)
- [10.2 Symmetry in Protein Assemblies](https://proteindesign.academy/module/10/2)
- [10.3 Interface Design Principles](https://proteindesign.academy/module/10/3)
- [10.4 Evaluating Assemblies](https://proteindesign.academy/module/10/4)
- [10.integration Run AlphaFold-Multimer](https://proteindesign.academy/module/10/integration)
- [What does this assembly actually form?](https://proteindesign.academy/module/10/capstone)
- [Use the Tools](https://proteindesign.academy/module/10/tools)
- [Start Here](https://proteindesign.academy/start-here)
- [Tools & Papers](https://proteindesign.academy/tools-and-papers)
- [Continuing Practice](https://proteindesign.academy/continuing-practice)